ApoSENSOR ATP Cell Viability assay kit product blog
Tags: Assay Kit; ApoSENSOR ATP Cell Viability; ApoSENSOR ATP Cell Viability assay kit;
The ApoSENSOR ATP Cell Viability n/a (Catalog #MBS841642) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the ApoSENSOR ATP Cell Viability Bioluminescence Assay Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Product Information: ApoSENSORTM Cell Viability Assay Kit: Bioluminescent Assay for Rapid Screening of Apoptosis and Cell Proliferation Simultaneously in Mammalian Cells. Simple & Convenient. Kit Size: 200 & 1000 Assays.
Background: Cell death (especially apoptosis) is an energy-dependent process that requires ATP. As ATP levels fall to a point where the cell can no longer perform basic metabolic functions, the cell will die. A typical apoptotic cell exhibits a significant decrease in ATP level. Therefore, loss of ATP level in cell has been used as an indicator of cell death. In contrast, cell proliferation has been recognized by increased levels of ATP. The ApoSENSOR Cell Viability Assay Kit utilizes bioluminescent detection of the ATP levels for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is extremely sensitive (detects 10-100 mammalian cells/well). The high sensitivity of this assay has led to many other applications for detecting ATP production in various enzymatic reactions, as well as for detecting low level bacterial contamination in samples such as blood, milk, urine, soil, and sludge.
Summary: Detection method- Luminometer or Beta Counter.
Species reactivity- Mammalian
Kit size- 200, 1000 assays
Applications- Bioluminescent detection of the ATP level via luciferase catalyzed reaction for a rapid screening of apoptosis and cell viability in mammalian cells.
Samples: Cell and tissue lysates, culture media, urine, soil, sludge, plasma and serum, as well as many other biological fluids. Features & Benefits: Simple one-step procedure; takes only 30 minutes
Fast and convenient
The assay can be done directly in culture plates requiring no harvest/washing/or sample preparations. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).