|The Goat APOE apoe (Catalog #MBS707198) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS707198 ELISA Kit recognizes Goat (Caprine, General) APOE.
The APOE apoe product has the following accession number(s) (GI #3913070) (NCBI Accession #Q28502.1) (Uniprot Accession #Q28502). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with APOE. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for APOE. The competitive inhibition reaction is launched between with pre-coated APOE and APOE in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of APOE in the samples. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma, tissue homogenates.
Assay Type: Competitive
Detection Range: 31.25 ng/ml -2000 ng/ml.
Sensitivity: 7.81 ng/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Goat Apolipoprotein E, APOE ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Digestive, Lens, Other tissues are correlated with this protein. APOE also interacts with the following gene(s): ABCA1, APOB, ESR2, LDLR, LPL, LRP1, SCARB1, VLDLR.