APAF1 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; APAF1 elisa kit; APAF1; Apoptosis Protease Activating Factor 1;
The Human APAF1 n/a (Catalog #MBS762798) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS762798 ELISA Kit recognizes Human APAF1.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Apoptosis Protease Activating Factor 1 ELISA Kit with the following immunoassay(s):
Typical Testing Data
Typical Standard Curve
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human APAF1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human APAF1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human APAF1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human APAF1 can be calculated.
Samples: Serum, plasma and other biological fluids.
Assay Type: Sandwich ELISA, Double Antibody
Detection Range: 0.156-10ng/ml
Sensitivity: 0.094ng/ml. Human Apoptosis Protease Activating Factor 1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.