|The ANXA5 anxa5 (Catalog #MBS224964) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s ANNEXIN V can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the ANXA5 anxa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ANXA5 anxa5 product has the following accession number(s) (GI #113960) (NCBI Accession #P08758.2) (Uniprot Accession #P08758). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual
for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the ANNEXIN V: Biotin ASSAY KIT with the following immunoassay(s):
Dot Blot (WB) (Dot-plot showing Ramos cells staining with Annexin V:FITC (ANNEX100F) versus Propidium Iodide resulting in three distinct populations)
Testing Data (Published customer image: Annexin V:FITC Assay Kit used for cell cycle analysis of cells treated with Arsenic trioxide and Macuna macrocarpa extract by flow cytometry.Image caption:Annexin V-FITC/propidium iodide (PI) analyses of arsenic trioxide (ATO)- and/or crude methanolic extract of Mucuna macrocarpa (CMEMM)-treated cells. HL-60 (a) and Jurkat (b) cells (1 x 105?cells/mL) were first treated with 5?mM N-acetyl cysteine (NAC), 50?μM butylated hydroxytoluene (BHT), or 40?μM alpha-tocopherol (VitE) or untreated, followed by treatment with 0.1% DMSO (CTL), 2.5?μM ATO, and/or 50?μg/mL CMEMM as indicated for 24?h. Quantitative percentages of apoptotic cells of ATO/CMEMM-treated cells were measured by flow cytometry. Data represent the result from one of three independent experiments.From: Lu KH, Lee HJ, Huang ML, Lai SC, Ho YL, Chang YS, Chi CW. Synergistic Apoptosis-Inducing Antileukemic Effects of Arsenic Trioxide and Mucuna macrocarpa Stem Extract in Human Leukemic Cells via a Reactive Oxygen Species-Dependent Mechanism.Evid Based Complement Alternat Med. 2012;2012:921430.)
This test employs the property of Annexin V to bind to the membrane phospholipid phosphatidylserine (PS) in the presence of Ca2+. PS is exposed at the cell surface during the early stages of apoptosis. Detection of PS is a very sensitive method for detecting cells entering apoptosis, at a time point considerably ahead of nuclear changes such as DNA degradation. The conjugation protocol used to prepare this product has not changed the native phospholipid binding properties of Annexin V. This protocol is designed to measure apoptosis easily and quickly in a sample of suspended cells.
Preservative Stabilisers: 0.02% Sodium Azide (NaN3), 1% Bovine Serum Albumin.