Anti-tissue transglutaminase IgG, IgG-tTG elisa kit product blog
Tags: ELISA Kit; Rat ELISA Kit; Anti-tissue transglutaminase IgG, IgG-tTG; Anti-tissue transglutaminase IgG, IgG-tTG elisa kit;
The Rat Anti-tissue transglutaminase IgG, IgG-tTG n/a (Catalog #MBS7212793) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS7212793 ELISA Kit recognizes Rat Anti-tissue transglutaminase IgG, IgG-tTG.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Anti-tissue transglutaminase IgG, IgG-tTG ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This TTG-IgG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat TTG-IgG.
Principle of the Assay: TTG-IgG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TTG-IgG antibody and an TTG-IgG-HRP conjugate. The assay sample and buffer are incubated together with TTG-IgG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TTG-IgG concentration since TTG-IgG from samples and TTG-IgG-HRP conjugate compete for the anti-TTG-IgG antibody binding site. Since the number of sites is limited, as more sites are occupied by TTG-IgG from the sample, fewer sites are left to bind TTG-IgG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TTG-IgG concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Quantitative Competitive
Sensitivity: 0.1 ug/mL. Rat Anti-tissue transglutaminase IgG, IgG-tTG ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.