Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow anti-LC1 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • C6orf103 elisa kit blog
 • CTNND2 elisa kit blog
 • KRT6A elisa kit blog
 • TUBbeta3 elisa kit blog
 • C1INH elisa kit blog
 • HE-4 elisa kit blog
 • BCL3 elisa kit blog
 • FANCI elisa kit blog
 • FP-S elisa kit blog
 • TPS elisa kit blog
 • ZNF277 elisa kit blog
 • RESISTIN elisa kit blog
 • CHD5 elisa kit blog
 • HYOU1 elisa kit blog
 • GC elisa kit blog
 • Rpl13 elisa kit blog
 • NRSN2 elisa kit blog
 • COL10 elisa kit blog
 • bFGF/FGF2 elisa kit blog
 • ABCG5 elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • beta-Hydroxybutyric Acid (beta-OHB) ELISA Kit
 • Donkey anti Goat IgG Secondary Antibody
 • PTCH1 Antibody
 • E-Selectin Western Blot Control
 • Endothelial Nitric Oxide Synthase 3 (ENOS-3) ELISA Kit
 • 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 Antibody
 • TNFRSF13B Recombinant Protein
 • PRF1 Antibody
 • Extracellular Matrix Protein 1 (ECM1) ELISA Kit
 • B cell activation factor, BAFF (TNFSF13B) ELISA Kit
 • Podocin (PDCN) Antibody
 • Tyrosine 3-Tryptophan 5 Monooxygenase Activation Protein Zet... ELISA Kit
 • EGFR (Epidermal Growth Factor Receptor) Antibody
 • TGF beta 2 Recombinant Protein
 downarrow more ...
separator

anti-LC1 elisa kit product blog

separator
Posted on 2015-09-15 17:16:08 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Sheep ELISA Kit; Anti-liver cytosol antibody type 1; anti-LC1 elisa kit; anti-LC1;
separator
The Sheep LC1 n/a (Catalog #MBS734338) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS734338 ELISA Kit recognizes Sheep (Ovine, General) LC1.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Sheep Anti-liver cytosol antibody type 1 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) LC1.

Principle of the Assay: anti-LC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing LC1 antigen and an anti-LC1-HRP conjugate. The assay sample and buffer are incubated together with anti-LC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the anti-LC1 concentration since anti-LC1 from samples and anti-LC1-HRP conjugate compete for the LC1 antigen binding site. Since the number of sites is limited, as more sites are occupied by anti-LC1 from the sample, fewer sites are left to bind anti-LC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The anti-LC1 concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 ng/mL. Intended Uses: This anti-LC1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep anti-LC1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Sheep Anti-liver cytosol antibody type 1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions