anti-IgA antibody elisa kit product blog
Tags: ELISA Kit; Bovine ELISA Kit; Anti-IgA Antibody; anti-IgA antibody elisa kit;
The Bovine anti-IgA antibody n/a (Catalog #MBS7202752) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS7202752 ELISA Kit recognizes Bovine anti-IgA antibody.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Bovine anti-IgA antibody ELISA Kit with the following immunoassay(s):
Typical Standard Curve/Testing Data
Intended Uses: This IgA-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine IgA-Ab. This ELISA kit for research use only!
Principle of the Assay: IgA-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing an IgA and an IgA-Ab-HRP conjugate. The assay sample and buffer are incubated together with IgA-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IgA-Ab concentration since IgA-Ab from samples and IgA-Ab-HRP conjugate compete for the IgA binding site. Since the number of sites is limited, as more sites are occupied by IgA-Ab from the sample, fewer sites are left to bind IgA-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgA-Ab concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Quantitative Competitive
Sensitivity: 1.0 ng/mL. Bovine anti-IgA antibody ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.