|The Bovine GM2 Ab IgM n/a (Catalog #MBS755965) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS755965 ELISA Kit recognizes Bovine (Cow, Cattle, General) GM2 Ab IgM.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Bovine Anti-GM2 (ganglioside) Antibody IgM ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: Anti-GM2 (ganglioside) Antibody IgM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Anti-GM2 (ganglioside) Antibody IgM antibody and an Anti-GM2 (ganglioside) Antibody IgM-HRP conjugate. The assay sample and buffer are incubated together with Anti-GM2 (ganglioside) Antibody IgM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-GM2 (ganglioside) Antibody IgM concentration since Anti-GM2 (ganglioside) Antibody IgM from samples and Anti-GM2 (ganglioside) Antibody IgM-HRP conjugate compete for the anti-Anti-GM2 (ganglioside) Antibody IgM antibody binding site. Since the number of sites is limited, as more sites are occupied by Anti-GM2 (ganglioside) Antibody IgM from the sample, fewer sites are left to bind Anti-GM2 (ganglioside) Antibody IgM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-GM2 (ganglioside) Antibody IgM concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 ng/mL. Bovine Anti-GM2 (ganglioside) Antibody IgM ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.