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Anti-GM2 Ab IgM elisa kit product blog

Posted on 2015-09-07 23:29:36 by mybiosource_staff
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Tags: ELISA Kit; Bovine ELISA Kit; Anti-GM2 (ganglioside) Antibody IgM; Anti-GM2 Ab IgM elisa kit; Anti-GM2 Ab IgM;
The Bovine GM2 Ab IgM n/a (Catalog #MBS755965) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS755965 ELISA Kit recognizes Bovine (Cow, Cattle, General) GM2 Ab IgM.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Bovine Anti-GM2 (ganglioside) Antibody IgM ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) GM2 Ab IgM.

Principle of the assay: Anti-GM2 (ganglioside) Antibody IgM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Anti-GM2 (ganglioside) Antibody IgM antibody and an Anti-GM2 (ganglioside) Antibody IgM-HRP conjugate. The assay sample and buffer are incubated together with Anti-GM2 (ganglioside) Antibody IgM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-GM2 (ganglioside) Antibody IgM concentration since Anti-GM2 (ganglioside) Antibody IgM from samples and Anti-GM2 (ganglioside) Antibody IgM-HRP conjugate compete for the anti-Anti-GM2 (ganglioside) Antibody IgM antibody binding site. Since the number of sites is limited, as more sites are occupied by Anti-GM2 (ganglioside) Antibody IgM from the sample, fewer sites are left to bind Anti-GM2 (ganglioside) Antibody IgM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-GM2 (ganglioside) Antibody IgM concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 ng/mL. Bovine Anti-GM2 (ganglioside) Antibody IgM ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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