|The Monkey anti-double stranded DNA, dsDNA IgG n/a (Catalog #MBS7225215) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS7225215 ELISA Kit recognizes Monkey (Rhesus Macaque, Rhesus Monkey, General) anti-double stranded DNA, dsDNA IgG.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Monkey anti-double stranded DNA, dsDNA IgG ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This dsDNA-IgG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey dsDNA-IgG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: dsDNA-IgG ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-dsDNA-IgG antibody and an dsDNA-IgG-HRP conjugate. The assay sample and buffer are incubated together with dsDNA-IgG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the dsDNA-IgG concentration since dsDNA-IgG from samples and dsDNA-IgG-HRP conjugate compete for the anti-dsDNA-IgG antibody binding site. Since the number of sites is limited, as more sites are occupied by dsDNA-IgG from the sample, fewer sites are left to bind dsDNA-IgG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The dsDNA-IgG concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Quantitative Competitive
Sensitivity: 1.0 ng/mL. Monkey anti-double stranded DNA, dsDNA IgG ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.