Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow ANP elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • CLN5 elisa kit blog
 • Nkap elisa kit blog
 • CEA elisa kit blog
 • TAF2 elisa kit blog
 • ORM elisa kit blog
 • SLPI elisa kit blog
 • TBG elisa kit blog
 • TLL1 elisa kit blog
 • CIT elisa kit blog
 • TESTO elisa kit blog
 • PDAP1 elisa kit blog
 • ALPPL2 elisa kit blog
 • CSN1S2B elisa kit blog
 • FTH elisa kit blog
 • GLCCI1 elisa kit blog
 • LRP1 elisa kit blog
 • ROCK Activity immunoblot kit blog
 • RSPH10B elisa kit blog
 • Cy.C elisa kit blog
 • CXCR2 elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • IgD Antibody
 • E Coli 0157:H7 Antibody
 • Corticosteroid Binding Globulin (CBG) Recombinant Protein
 • Carcinoembryonic Antigen (CEA) (CEACAM6) Antibody
 • SSX4 cDNA Clone
 • CD45 Antibody
 • activation-induced cytidine deaminase (AICDA) Antibody
 • Histone H3 Antibody
 • Cell adhesion molecule 3 (Cadm3) ELISA Kit
 • Apolipoprotein E (APOE) ELISA Kit
 • Growth Hormone Releasing Hormone (GHRH) ELISA Kit
 • Insulin Receptor Substrate 2 (IRS-2) ELISA Kit
 • IL16 Antibody
 • cereblon (CRBN) ELISA Kit
 downarrow more ...
separator

ANP elisa kit product blog

separator
Posted on 2015-11-26 03:36:46 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Goat ELISA Kit; Atrial Natriuretic Peptide; ANP elisa kit; ANP;
separator
The Goat ANP nppa (Catalog #MBS739707) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS739707 ELISA Kit recognizes Goat (Caprine, General) ANP.

The ANP nppa product has the following accession number(s) (GI #178638) (NCBI Accession #AAA35529.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Goat Atrial Natriuretic Peptide ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) ANP.

Principle of the Assay: ANP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANP antibody and an ANP-HRP conjugate. The assay sample and buffer are incubated together with ANP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANP concentration since ANP from samples and ANP-HRP conjugate compete for the anti-ANP antibody binding site. Since the number of sites is limited, as more sites are occupied by ANP from the sample, fewer sites are left to bind ANP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANP concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 pg/mL. Intended Uses: This ANP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat ANP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Goat Atrial Natriuretic Peptide ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Arrhythmias, Cardiac, Cardiomegaly, Cardiomyopathies, Heart Diseases, Heart Failure, Hypertension, Hypertrophy, Hypertrophy, Left Ventricular, Kidney Diseases, Myocardial Ischemia are some of the diseases may be linked to Goat Atrial Natriuretic Peptide ELISA Kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions