AngII elisa kit product blog
Tags: ELISA Kit; Rat ELISA Kit; Angiotensin II (AngII); AngII; AngII elisa kit;
The Rat AngII n/a (Catalog #MBS450632) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS450632 ELISA Kit recognizes Rat AngII.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Angiotensin II (AngII) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of AngII in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AngII. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to AngII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AngII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of AngII in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samples: Rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 62.5-4000pg/mL
Sensitivity: <24.31pg/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AngII were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AngII were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Rat Angiotensin II (AngII) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.