Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Assay Kit Blog  rightarrow ANG elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • GC elisa kit blog
 • RPN2 elisa kit blog
 • TTR elisa kit blog
 • BTG3 elisa kit blog
 • MHC 1 elisa kit blog
 • VDAC-1 elisa kit blog
 • LAT elisa kit blog
 • DCN elisa kit blog
 • SULF1 clia kit blog
 • IFNalpha2 elisa kit blog
 • DDX19A elisa kit blog
 • PRO-NGF1 elisa kit blog
 • Lyme IgG elisa kit blog
 • PKIalpha elisa kit blog
 • CBX3 elisa kit blog
 • PTPN1 elisa kit blog
 • Trastuzumab (Herceptin) elisa kit blog
 • CD5L elisa kit blog
 • IL-2 elisa kit blog
 • C6orf57 elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • GNPAT Antibody
 • C3c Antibody
 • HLA class I histocompatibility antigen, alpha chain G (HLA-G... ELISA Kit
 • MBD2 Peptide
 • 14 kDa phosphohistidine phosphatase (PHPT1) ELISA Kit
 • Defensin Beta 2 (DEFb2) ELISA Kit
 • ADH1B Antibody
 • Breast cancer metastasis-suppressor 1 (BRMS1) Recombinant Protein
 • P-Selectin (SELP) ELISA Kit
 • SH3-domain GRB2-like endophilin B2 (SH3GLB2) ELISA Kit
 • HTLV-1 gp21 Recombinant Protein
 • 60 kDa lysophospholipase (Aspg) Recombinant Protein
 • Insulin Receptor beta-Y1189 (INSR) Antibody
 downarrow more ...

ANG elisa kit product blog

Posted on 2017-11-01 13:29:39 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Sheep ELISA Kit; Angiogenin; ANG elisa kit; ANG;
The Sheep ANG ang (Catalog #MBS741402) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS741402 ELISA Kit recognizes Sheep (Ovine, General) ANG.

The ANG ang product has the following accession number(s) (GI #178250) (NCBI Accession #AAA51678.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Sheep Angiogenin ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) ANG.

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This ANG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep ANG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

PRINCIPLE OF THE ASSAY ANG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANG antibody and an ANG-HRP conjugate. The assay sample and buffer are incubated together with ANG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANG concentration since ANG from samples and ANG-HRP conjugate compete for the anti-ANG antibody binding site. Since the number of sites is limited, as more sites are occupied by ANG from the sample, fewer sites are left to bind ANG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANG concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 pg/mL. Sheep Angiogenin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions