AMPK elisa kit product blog
Tags: ELISA Kit; Mouse ELISA Kit; AMPK elisa kit; AMPK; Phosphorylated Adenosine Monophosphate Activated Protein Kinase;
The Mouse AMPK n/a (Catalog #MBS760308) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS760308 ELISA Kit recognizes Mouse AMPK.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Phosphorylated Adenosine Monophosphate Activated Protein Kinase ELISA Kit with the following immunoassay(s):
Typical Testing Data
Typical Standard Curve
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse AMPK antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse AMPK antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse AMPK amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse AMPK can be calculated.
Samples: Serum, plasma and other biological fluids.
Assay Type: Sandwich ELISA, Double Antibody
Detection Range: 0.313-20ng/ml
Sensitivity: 0.188ng/ml. Mouse Phosphorylated Adenosine Monophosphate Activated Protein Kinase ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.