ALT elisa kit product blog
Tags: ELISA Kit; Monkey ELISA Kit; ALT elisa kit; ALT;
The Monkey ALT n/a (Catalog #MBS2512422) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2512422 ELISA Kit recognizes Monkey ALT.The ALT n/a product has the following accession number(s) (GI #13786560) (NCBI Accession #NP_112692.1) (Uniprot Accession #Q9AZ68). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey ALT concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey ALT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey ALT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey ALT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Monkey ALT. You can calculate the concentration of Monkey ALT in the samples by comparing the OD of the samples to the standard curve.
Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 0.31-20ng/mL
Sensitivity: 0.19ng/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Monkey ALT were tested 20 times on one plate, respectively.
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Monkey ALT were tested on 3 different plates, 20 replicates in each plate. Monkey ALT (Alanine Transaminase) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.