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ALPP elisa kit product blog

Posted on 2016-06-18 04:59:32 by mybiosource_staff
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Tags: ELISA Kit; Human ELISA Kit; ALPP; ALPP elisa kit; Alkaline Phosphatase, Placental (ALPP);
The Human ALPP alpp (Catalog #MBS2022597) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2022597 ELISA Kit recognizes Human (General) ALPP.

The ALPP alpp product has the following accession number(s) (GI #94721246) (NCBI Accession #NP_001623.3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Alkaline Phosphatase, Placental (ALPP) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
 Typical Testing Data/Standard Curve (for reference only) ALPP.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ALPP in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ALPP. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ALPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ALPP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ALPP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Samples: Serum, plasma, tissue homogenates and other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 125-8,000pg/mL
Sensitivity: <50pg/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ALPP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ALPP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Alkaline Phosphatase, Placental (ALPP) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. ALPP also interacts with the following gene(s): PTS. Adenocarcinoma, Breast Neoplasms, Colonic Neoplasms, Disorders of Sex Development, Kidney Diseases, Liver Diseases, Lung Diseases, Nervous System Diseases, Pain, Pregnancy Complications are some of the diseases may be linked to Alkaline Phosphatase, Placental (ALPP) ELISA Kit. Amniotic Fluid, Heart, Intestine, Placenta, Uterus tissues are correlated with this protein. The following patways have been known to be associated with this gene.
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