Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow AFGF-1 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • MLYCD elisa kit blog
 • ASM elisa kit blog
 • MT2 elisa kit blog
 • OR10W1 elisa kit blog
 • CTNT elisa kit blog
 • ZUN elisa kit blog
 • pre-CFB elisa kit blog
 • sICAM-1/CD54 elisa kit blog
 • TRAPPC6A elisa kit blog
 • CD36 elisa kit blog
 • STM elisa kit blog
 • H-FABP elisa kit blog
 • ERalpha elisa kit blog
 • RXR elisa kit blog
 • HNF4G elisa kit blog
 • Anguilla anguilla (European Eel) pcr kit blog
 • SHROOM4 elisa kit blog
 • APOE elisa kit blog
 • ATGA/TGAB elisa kit blog
 • IL-18 elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • IA2 Recombinant Protein
 • Protein-arginine deiminase type-2 (PADI2) Antibody
 • HERPES SIMPLEX VIRUS 2 Antigen
 • Legionella pneumophila antigen, LP Ag ELISA Kit
 • AGTRAP cDNA Clone
 • Calreticulin-3 (CALR3) Antibody
 • CTNNBL1 Antibody
 • Mycobacterium tuberculosis Immunogenic protein MPT64 Antibody
 • Dopachrome Tautomerase (DT) ELISA Kit
 • 5-LO ELISA Kit
 • Anti-Type II Collagen Antibody (CII) ELISA Kit
 • Lactadherin (MFGE8) Recombinant Protein
 • BMYB-MYBL2 Antibody
 • Synaptosomal Associated Protein 25kDa (SNAP25) ELISA Kit
 downarrow more ...
separator

AFGF-1 elisa kit product blog

separator
Posted on 2015-09-10 18:52:15 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Goat ELISA Kit; Acidic fibroblast growth factor 1; AFGF-1 elisa kit; AFGF-1;
separator
The Goat AFGF-1 fgf1 (Catalog #MBS750511) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS750511 ELISA Kit recognizes Goat (Caprine, General) AFGF-1.

The AFGF-1 fgf1 product has the following accession number(s) (GI #13236891) (NCBI Accession #AAB29057.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Goat Acidic fibroblast growth factor 1 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) AFGF-1.

Principle of the Assay: AFGF-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AFGF-1 antibody and an AFGF-1-HRP conjugate. The assay sample and buffer are incubated together with AFGF-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AFGF-1 concentration since AFGF-1 from samples and AFGF-1-HRP conjugate compete for the anti-AFGF-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AFGF-1 from the sample, fewer sites are left to bind AFGF-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AFGF-1 concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive. Intended Uses: This AFGF-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat AFGF-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications
!Intended Uses: This AFGF-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat AFGF-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Goat Acidic fibroblast growth factor 1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions