AFB1 elisa kit product blog
Tags: ELISA Kit; Aflatoxin B1; AFB1 elisa kit; AFB1;
The Aflatoxin B1 afb1 (Catalog #MBS702574) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase.The Aflatoxin B1 afb1 product has the following accession number(s) (GI #170677210) (NCBI Accession #ACB30677.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Aflatoxin B1 antigen. Standards or samples are added to the appropriate microtiter plate wells with Aflatoxin B1 specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Aflatoxin B1 and Aflatoxin B1 in standards or samples with the Aflatoxin B1 special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Aflatoxin B1 in the standards or samples. The color development is stopped and the intensity of the color is measured.
Background/Introduction: Aflatoxins are toxic metabolites of the fungi species, such as Aspergillus flavus and Aspergillus parasiticus. Aflatoxins have a strong carcinogenicity, commonly found in cereal, nuts, cotton seed, human blood and animal feed. Aflatoxin B1 is mainly natural Aflatoxins contamination. Aflatoxins are listed as Category I carcinogen with strong carcinogenicity by the International Agency for Research on Cancer in 1993. Contamination can occur in the field, during growing, harvest, or in processing, storage and transport, detect timely pollution sources is the best way to prevent aflatoxin contamination. This kit is a detection product developed based on ELISA technology, with operation time as short as 25 min and a sensitivity of 0.15 ug/kg, and linear range from 0.15 ug/kg to 4.05 ug/kg.
Samples: Feedstuff, feed, grain, edible oil
Assay Type: Quantitative Competitive
Detection Range: 0.15 ug/kg~4.05 ug/kg
Sensitivity: < 0.15 ug/kg. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<10%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Aflatoxin B1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.