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ADORA2b elisa kit product blog

Posted on 2017-10-04 11:43:38 by mybiosource_staff
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Tags: ELISA Kit; Rat ELISA Kit; Adenosine A2b Receptor; ADORA2B; ADORA2B elisa kit;
The Rat ADORA2b adora2b (Catalog #MBS762150) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS762150 ELISA Kit recognizes Rat (Rattus, General) ADORA2b.

The ADORA2b adora2b product has the following accession number(s) (GI #178150) (NCBI Accession #AAA51598.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Adenosine A2b Receptor ELISA Kit with the following immunoassay(s):
Typical Testing Data
Typical Testing Data ADORA2b.

Typical Standard Curve
Typical Standard Curve ADORA2b.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat ADORA2b antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat ADORA2b antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat ADORA2b amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofRat ADORA2b can be calculated.

Samples: Serum, plasma and other biological fluids.
Assay Type: Sandwich ELISA, Double Antibody
Detection Range: 0.313-20ng/ml
Sensitivity: 0.188ng/ml. Rat Adenosine A2b Receptor ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Ascites, Blood, Brain, Eye, Heart, Intestine, Lung, Placenta, Prostate, Stomach tissues are correlated with this protein. ADORA2b also interacts with the following gene(s): ADCY9. The following patways have been known to be associated with this gene. Cardiovascular Diseases, Disease Models, Animal, Edema, Fibrosis, Heart Diseases, Hypertension, Inflammation, Kidney Diseases, Lung Injury, Nervous System Diseases are some of the diseases may be linked to Rat Adenosine A2b Receptor ELISA Kit.
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