ACH elisa kit product blog
Tags: ELISA Kit; ACH elisa kit; ACH; Acetylcholine (ACH); General ELISA Kit;
The General ACH n/a (Catalog #MBS2700397) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2700397 ELISA Kit recognizes General ACH.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the General Acetylcholine (ACH) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of acetylcholine in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to acetylcholine has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled acetylcholine and unlabeled acetylcholine (Standards or samples) with the pre-coated antibody specific to acetylcholine. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of acetylcholine in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of acetylcholine in the sample.
Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Competitive
Detection Range: 12.35-1,000pg/mL
Sensitivity: < 5.31pg/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level acetylcholine were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level acetylcholine were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%. General Acetylcholine (ACH) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.