Ab42 clia kit product blog
Tags: CLIA Kit; Rat CLIA Kit; Abeta42 (Amyloid Beta 42); Ab42 clia kit; Ab42;
The Rat Ab42 n/a (Catalog #MBS2531913) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2531913 CLIA Kit recognizes Rat Ab42.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of Abeta42 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Abeta42. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Abeta42 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Abeta42, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Abeta42. You can calculate the concentration of Abeta42 in the samples by comparing the RLU value of the samples to the standard curve.
Assay Type: Sandwich
Detection Range: 7.813-500pg/mL
Sensitivity: Min: 4.688pg/mL; Max: 500pg/mL.