Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Assay Kit Blog  rightarrow AA-NAT elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • RHOA elisa kit blog
 • MALT1 elisa kit blog
 • PS elisa kit blog
 • ANKRD24 elisa kit blog
 • COL15 elisa kit blog
 • ALB elisa kit blog
 • LPGAT1 elisa kit blog
 • E2F2 elisa kit blog
 • EPHA10 elisa kit blog
 • INA elisa kit blog
 • GC elisa kit blog
 • ADCY3 elisa kit blog
 • ACAT1 elisa kit blog
 • ADP elisa kit blog
 • KLK 1 elisa kit blog
 • Lptn elisa kit blog
 • HP elisa kit blog
 • PG-E1 elisa kit blog
 • GATA4 elisa kit blog
 • sPLA2 elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • H15 (A-Australia-83-2000)(H15N8 ) Antibody
 • GAPDH Antibody
 • SR-B1 Antibody
 • Lysolecithin ELISA Kit
 • LELP1 Antibody
 • CyPB Antibody
 • POU2F1 Antibody
 • neuropilin 1 (NRP1) ELISA Kit
 • wingless-type MMTV integration site family, member 7B (WNT7B... ELISA Kit
 • CD105-Endoglin Antibody
 • ADAMTS17 Antibody
 • Cathepsin K (CTSK) Recombinant Protein
 • GOLGA3 Antibody
 downarrow more ...

AA-NAT elisa kit product blog

Posted on 2015-12-06 02:13:02 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Canine ELISA Kit; Arylalkylamine N-acetyltransferase; AA-NAT elisa kit; AA-NAT;
The Canine AA-NAT n/a (Catalog #MBS734331) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS734331 ELISA Kit recognizes Canine (Dog, General) AA-NAT.

The AA-NAT n/a product has the following accession number(s) (GI #160333514) (NCBI Accession #NP_001073122.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Canine Arylalkylamine N-acetyltransferase ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) AA-NAT.

Principle of the Assay: AA-NAT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AA-NAT antibody and an AA-NAT-HRP conjugate. The assay sample and buffer are incubated together with AA-NAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AA-NAT concentration since AA-NAT from samples and AA-NAT-HRP conjugate compete for the anti-AA-NAT antibody binding site. Since the number of sites is limited, as more sites are occupied by AA-NAT from the sample, fewer sites are left to bind AA-NAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AA-NAT concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 5.0-100 U/mL
Sensitivity: 1.0u/mL. Intended Uses: This AA-NAT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine AA-NAT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Canine Arylalkylamine N-acetyltransferase ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions