8-iso-PGF2alpha elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; 8-iso-PGF2alpha (8-isoprostane); 8-iso-PGF2alpha elisa kit; 8-iso-PGF2alpha;
The Human 8-iso-PGF2alpha n/a (Catalog #MBS7606825) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS7606825 ELISA Kit recognizes Human 8-iso-PGF2alpha.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human 8-iso-PGF2alpha (8-isoprostane) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with 8-iso-PGF2alpha. During the reaction, 8-iso-PGF2alpha in the sample or standard competes with a fixed amount of 8-iso-PGF2alpha on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 8-iso-PGF2alpha. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzymesubstrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 8-iso-PGF2alpha in the samples is then determined by comparing the O D of the samples to the standard curve.
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Assay Type: Competitive
Detection Range: 15.625-1000pg/ml
Sensitivity: <9.375pg/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 8-iso-PGF2alpha were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 8-iso-PGF2alpha were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%. Human 8-iso-PGF2alpha (8-isoprostane) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.