|The Mouse 4-HNE n/a (Catalog #MBS2533505) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2533505 ELISA Kit recognizes Mouse (Murine, General) 4-HNE.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual
for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse 4-HNE (4-Hydroxynonenal) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Mouse 4-HNE concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse 4-HNE. During the reaction, Mouse 4-HNE in the sample or standard competes with a fixed amount of Mouse 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse 4-HNE. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the Substrate Reagent is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change can be measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Mouse 4-HNE in samples can be calculated by comparing the OD of the samples with the standard curve.
Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Competitive
Detection Range: 1.56-100 ng/mL
Sensitivity: 0.94 ng/mL. Mouse 4-HNE (4-Hydroxynonenal) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.