|The Human 27 OHC n/a (Catalog #MBS7606791) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS7606791 ELISA Kit recognizes Human (General) 27 OHC.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual
for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human 27 OHC (27-Hydroxycholesterol) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 27 OHC. During the reaction, 27 OHC in the sample or standard competes with a fixed amount of 27 OHC on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 27 OHC. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 27 OHC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samples: Serum, plasma and other biological fluids.
Assay Type: Competitive ELISA, Coated with Antigen
Detection Range: 15.625-1000ng/ml
Sensitivity: 9.375ng/ml. Human 27 OHC (27-Hydroxycholesterol) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.