|The Sheep 15-S-HETE n/a (Catalog #MBS756149) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS756149 ELISA Kit recognizes Sheep (Ovine, General) 15-S-HETE.
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Principle of the assay: 15-S-HETE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-15-S-HETE antibody and an 15-S-HETE-HRP conjugate. The assay sample and buffer are incubated together with 15-S-HETE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 15-S-HETE concentration since 15-S-HETE from samples and 15-S-HETE-HRP conjugate compete for the anti-15-S-HETE antibody binding site. Since the number of sites is limited, as more sites are occupied by 15-S-HETE from the sample, fewer sites are left to bind 15-S-HETE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 15-S-HETE concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 nmol/L. Sheep 15 (S) -Hydroxyeicosatetraenoic Acid ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.