12-HETE elisa kit product blog
Tags: ELISA Kit; Monkey ELISA Kit; 12-HETE elisa kit; 12-HETE;
The Monkey 12-HETE n/a (Catalog #MBS2505578) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2505578 ELISA Kit recognizes Monkey 12-HETE.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey 12-HETE concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 12-HETE. During the reaction, 12-HETE in the sample or standard competes with a fixed amount of 12-HETE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 12-HETE. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of 12-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.
Samples: Serum, Plasma, Biological Fluids
Assay Type: Competitive
Detection Range: 0.781-50ng/mL
Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL. Monkey 12-HETE (12-Hydroxyeicosatetraenoic Acid) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.