11-Deoxycortisol elisa kit product blog
Tags: ELISA Kit; Monkey ELISA Kit; 11-Deoxycortisol; 11-Deoxycortisol elisa kit;
The Monkey 11-Deoxycortisol n/a (Catalog #MBS7219925) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS7219925 ELISA Kit recognizes Monkey 11-Deoxycortisol.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Principle of the Assay: 11-Deoxycortisol ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-11-Deoxycortisol antibody and an 11-Deoxycortisol-HRP conjugate. The assay sample and buffer are incubated together with 11-Deoxycortisol-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 11-Deoxycortisol concentration since 11-Deoxycortisol from samples and 11-Deoxycortisol-HRP conjugate compete for the anti-11-Deoxycortisol antibody binding site. Since the number of sites is limited, as more sites are occupied by 11-Deoxycortisol from the sample, fewer sites are left to bind 11-Deoxycortisol-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 11-Deoxycortisol concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity: 1.0 pmol/mL.
Assay Type: Competitive. Intended Uses: This 11-Deoxycortisol ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey 11-Deoxycortisol. This ELISA kit for research use only, not for therapeutic applications!. Monkey 11-Deoxycortisol ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.